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Pavesi-Negri studied music and singing with Giovanni Quacquerini, Giovanni Rossi and Amilcare Ponchielli. He was made a master composer in the Academia Filarmonica of Bologna in 1877, and he wrote and published a biography of singer Rosmunda Pisaroni.
Pavesi-Negri lived in Florence, Italy for 36 years, where he concentrated his efforts as a singing teacher, producing some of the more accomplished students of his day, including Italo Cristalli, Angelo Masini, Amedeo Bassi, Enrico Ventura and Nunzio Rapisardi. Pavesi-Negri himself was also known to be a fine baritone singer who gave some beautiful performances during his lifetime.
Eventually he retired to his hometown of Piacenza, Italy, where he was a great support to the Musical High School there. He left his entire library to them after his death.